RNA structure analysis in human spliceosomes reveals a compact 3 D arrangement of snRNAs at the catalytic core
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RNA structure analysis of human spliceosomes reveals a compact 3D arrangement of snRNAs at the catalytic core.
Although U snRNAs play essential roles in splicing, little is known about the 3D arrangement of U2, U6, and U5 snRNAs and the pre-mRNA in active spliceosomes. To elucidate their relative spatial organization and dynamic rearrangement, we examined the RNA structure of affinity-purified, human spliceosomes before and after catalytic step 1 by chemical RNA structure probing. We found a stable 3-wa...
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تاریخ انتشار 2013